rDNA 1-1
R DNA
Recombinant DNA technology is joining DNA molecules - often from two different species - to produce genetic combinations that are value for science medicine agriculture industry etc. Largely result of work of Paul Berg, Daniel Nathan and others in 1970s
- Techniques for injection
- Electroporation
- Gene gun
- Nanoparticles
- Retrovirus or adenovirus
- Magnetofection
Diagram
- Selection and isolation of desired DNA sequence using restriction enzyme.
- Selection of suitable vector plasmid or virus
- Introduce desired DNA into vector.
- Insert vector into host cells like E coli bacteria
- Selection of transformed host cells.
- Multiplication of desired cells
Applications
- Gene structure mapping and function analysis
- Biosynthesis of insulin and growth hormones
- Agriculture nitrogen fixation
- Gene therapy and control genetic diseases ADA
- Diagnosis. Detect virus and bacteria.
- Cystic fibrosis and gene therapy application.
- Vaccine. Hepatitis B.
Limitations
- Safety precautions to avoid hazards
- Unknown effects in the long-term like cancer and biodiversity loss
- Ethical concerns like misuse and human genetic engineering
With crisper cas 9 technique it will be revolutionized.
Transgenic vs gene targeting
